An indirect ELISA was established with expressed3A protein of FMDV as coating antigen and was experimentally used to detect FMDV infection sera, immune sera and other disease sera. 用表达的口蹄疫3A蛋白作为包被抗原,建立了间接ELISA试验,对口蹄疫病毒感染血清、免疫血清和其它非口蹄疫病毒血清进行了检测研究。
The immune response profile following viral infection was analyzed by hemagglutination inhibition ( HI) test for both acute and convalescent phase sera of the confirmed cases. 血凝抑制(HI)实验检测急性期和恢复期血清,评价检测病毒感染后的免疫应答情况。
Clearing non-associated antibodies from immune sera against swine fever 免疫吸附去除猪瘟免疫血清中非相关抗体
Screening of Schistosoma japonicum Cercaria cDNA Library Using the Schistosoma japonicum Natural Molecular Vaccine Induced Immune Sera 抗日本血吸虫病天然分子疫苗免疫猪血清筛选日本血吸虫尾蚴cDNA文库结果分析
The detection of specific immune complex by using double McAb sandwich ELISA in sera of patients with neurocysticercosis 双单抗夹心ELISA法检测脑囊尾蚴病血清特异性免疫复合物
The differences of target antigens recognized by immune sera from patients infected with Plasmodium falciparum 恶性疟感染病人免疫血清识别靶抗原的差异
Using isolated immune complex ( IC) from sera of the patients with tuberculosis and artificial IC, complement mediated solubilizing capacity ( CMSC) in 72 TB patients was studied. 利用从患者体内分离的特异性免疫复合物(IC)和人工特异性IC对72例结核病患者补体介导的免疫复合物溶解能力(CMSC)进行了研究。
Detection and Characterization of Antigen Component of Circulating Immune Complex in Sera from Patients with Lung Cancer by Monoclonal and Polyclonal Antibodies 单克隆和多克隆抗体对肺癌患者血清循环免疫复合物抗原组份的检测和分析
Using a monoclonal antibody F1, two ELISA methods for detecting the circulating immune complexes and antigens in dissociates by 8M urea after PEG precipitation of sera were developed. 应用抗包虫单克隆抗体F1建立了检测包虫病人循环免疫复合物和解离抗原的ELISA法。
Detection Circulation Immune Complexes in Sera of Different Type Schistosomiasis Patient Using Polyethylene Glycol Precipitation 聚乙二醇沉淀试验检测不同类型日本血吸虫病患者血清循环免疫复合物
In this study, we purified the recombinant PDC-E2 expressed in E. coli. by using immune affinity chromatography method and identified its immune reactivity with sera of PBC patients by using Western blot analyses and ELISA. 本研究利用免疫亲和层析技术对PDC-E2的原核表达产物进行了纯化,并通过ELISA和免疫印迹试验对纯化蛋白进行了免疫活性鉴定。
The purified specific rabbit-anti-human TGF-β 1 polyclonal antibodies were obtained from the immune sera of rabbits, and it can react with human TGF-β 1. 获得初步纯化的兔抗人TGF-β1特异性多克隆抗体,能与人TGF-p;起反应。
Method: Use the methods of radiate immune and contrasting color to determine 36 cases of liver cancer patients and 30 cases of sera AFP, HA, SA level of healthy people. 方法:采用放射免疫法和比色法测定36例原发性肝癌病人和30例健康人血清AFP、HA、SA的水平。
Poliovirus was isolated from bile of 6 cases with biliary tract diseases. Identification of the isolated poliovirus strains with standard immune sera revealed that they all belonged to type ⅱ poliovirus. 从6例胆道疾患病人的胆汁中分离出脊髓灰质炎病毒,经用标准免疫血清中和试验,证实为Ⅱ型脊髓灰质炎病毒。
Micro-polyethylene glycol ( M-PEG) precipitation turbidimetry and solid phase Clq,-SPA-ELISA for detecting the circulating immune complexes in mink sera were established. 本试验建立了检测水貂循环免疫复合物(CIC)的微量PEG沉淀比浊法和固相C(1q)SPAELISA。
Conclusion The immune sera from pig vaccinated with the natural molecular vaccine can be used as a probe to screen for new genes in the Sj cercaria cDNA. 结论疫苗免疫猪血清可作为筛选日本血吸虫新基因的有效探针,新发现1个日本血吸虫相关基因。
Methods: A double mono-clonal antibody sandwich enzyme-linked immunosorbent assay ( ELISA) was used to detect the circulating antigens, antibodies and immune complex in 109 sera of neurocysticercosis. 方法:用ELISA法检测109例脑囊尾蚴病患者血清抗原、抗体及循环免疫复合物。
Methods Using recombinant PDC-E2, we established immune blotting test ( IBT) and enzyme-linked immunosorbent assay ( ELISA) to detect anti-M2 autoantibody in the sera of patients with PBC, compared with healthy groups, other liver diseases and other autoimmune diseases. 方法采用重组表达的PDC-E2建立了免疫印迹法(IBT)和酶联免疫吸附试验(ELISA)。检测40例PBC患者血清的M2抗体,以其他肝病患者、自身免疫病患者、健康体检者作对照。
Methods Inoculate the two kinds of tick-borne encephalitis vaccines into volunteers and observe the systemic and local reactions. Detect immune sera for neutralizing antibody titers by ELISA and plaque reduction neutralization test. 方法两种疫苗分别于接种后,观察局部反应和全身反应,免疫血清用酶联免疫吸附法(ELISA)和蚀斑减少试验法检测其中和抗体效价。
The inhibitory effects of immune sera against two recombinant hybrid antigens on the growth of Plasmodium falciparum in vitro 抗恶性疟重组复合抗原的免疫血清对恶性疟原虫体外生长的抑制作用
Agal-gel immunodiffusion were conducted with the sera of the control and the eight standard immune sera achieved by immunizing corresponding antigens to the mice. The results showed that antisera of glycinin and P-conglycinin showed no cross-reaction with P-conglycinin and glycinin antigens, respectively. 取免疫8种大豆提纯抗原所得的相应标准免疫血清及对照血清,对抗原与相应血清进行琼脂扩散试验,结果表明,大豆球蛋白的抗血清与β-伴大豆球蛋白无交叉反应;
Detection of Circulating Immune Complexes in Sera from Patients with Hepatitis of Various Types and Liver Cirrhosis 各型肝炎与肝硬化病人血清循环免疫复合物的检测
Circulating Immune Complexes in Sera of Patients with Ovarian Malignant Tumor 卵巢恶性肿瘤患者血清免疫复合物的测定
The immune rabbit sera were used for passive immunity test in mice. 并以家兔免疫血清在小鼠进行被动免疫保护实验。
No specific stain was found in human cardiac muscle tissue reacted with immune sera or normal sera. 结果发现,正常血清和免疫血清与心肌均未出现明显的特异性染色的痕迹。